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  Contact: Anne M. Stark
  Phone: (925) 422-9799
  E-mail: stark8@llnl.gov
  FOR IMMEDIATE RELEASE
June 4, 2007
NR-07-06-01

Researchers track how spores break out
of dormant state

LIVERMORE, Calif. – Tapping into the unknown world of awakening dormant bacterial spores, researchers have revealed through atomic force microscopy (AFM) the alterations of spore coat and germ cell wall that accompany the transformation from a spore to a vegetative cell.

spore progression
Emergence of vegetative cells: 60- to 70-nm-deep apertures in the rodlet layer that gradually enlarged (C and D), and subsequently eroded the entire spore coat (E). Germ cells emerged from these apertures.
Click for high-resolution image

When starved of nutrients Bacillus (rod-shapedbacteria) cells initiate a series of genetic,biochemical and structural events that resultin the formation of metabolically dormantspores.

They can remain dormant for extended periods and, partly because of their tough spore coat, have a significant resistance to extreme environmental factors including heat, radiation and toxic chemicals. However, once in favorable conditions, spores break the dormant state through germination and reenter the vegetative mode of replication.

Although significant progress has been madein understanding the biochemical and geneticbases of the spore germination process, itis still unclear how a spore breaks out ofits dormant state.

But a new in vitro study of singlegerminating Bacillus atrophaeus sporesdetails how the spore coat structures breakdown, and it shows with unprecedented resolutionhow the new bacterium emerges from the disintegratingspore.

The new research, led by Lawrence Livermore National Laboratory scientists, appears in the June 4 issue of the Proceedings of the National Academy of Sciences.

“A thorough understanding of sporegermination is important for the developmentof new countermeasures that identify theearliest stages of a wide range of sporemediated diseases, including botulism, gasgangrene and pulmonary anthrax,” saidAlexander Malkin, senior author from LLNL’sBiosciences and Biotechnology Division.

“Butit’s also important to gain fundamentalinsights into the key events in bacterialcell development.”

spores
Atomic force microscope (AFM) images of the rodlet layer of an intact spore (top), a degrading rodlet layer during germination (middle) and the cell wall structure of the newly emerging vegetative cell (bottom)
Click for high resolution image

The researchers, including Marco Plomp, leadauthor at LLNL, and those from Children’sHospital Oakland Research Institute and NorthwesternUniversity, used AFM to identify disassemblyof the outer spore coat rodlet structures,which appear to be structurally similar toamyloid fibrils that have been associatedwith neural degenerative diseases, such asAlzheimer’s and prion diseases.

“Theextreme physical and chemical resistanceof Bacillus spores suggests thatevolutionary forces have captured the mechanicalrigidity and resistance of these amyloidself-assembling biomaterials to structurethe protective outer spore surface,” Plompsaid.

When exposed to a solution that triggersgermination, nanometer sized etch pits wereseen developing in the rodlet layer. Theseetch pits evolved into ever widening fissures,leaving narrow strips of remaining rodletstructure. In the end, 1- to 3- nm-wide fibrilsremained. The in vitro AFM imagingalso revealed the porous fibrous cell wallstructure of newly emerging and mature vegetativecells, consisting of a network of nanometer-widepeptidoglycan fibers.

“These resultsshow that dynamic AFM is a promising toolto investigate the formation and evolutionof the bacterial cell wall,” Malkinsaid.

The research is funded by LLNL’s LaboratoryDirected Research and Development program,the Defense Advanced Research Project Agency(DARPA) and the Federal Bureau of Investigation.

Founded in 1952, Lawrence Livermore National Laboratory is a national security laboratory, with a mission to ensure national security and apply science and technology to the important issues of our time. Lawrence Livermore National Laboratory is managed by the University of California for the U.S. Department of Energy’s National Nuclear Security Administration.


Founded in 1952, Lawrence Livermore National Laboratory is a national security laboratory that develops science and engineering technology and provides innovative solutions to our nation's most important challenges. Lawrence Livermore National Laboratory is managed by Lawrence Livermore National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration.