Lawrence Livermore National Laboratory

08/08/2013


The microbial world of biomass deconstruction became more clear with a joint study, including a Lawrence Livermore National Laboratory (LLNL) co-author, of a thermophillic bacterial consortium adapted to switchgrass. This splatterplot is a visual representation of the consortium's metagenome. Image courtesy of Patrik D'haeseleer, LLNL and the Joint BioEnergy Institute

Microbial who-done-it for biofuels

Stephen P Wampler, LLNL, (925) 423-3107, wampler1@llnl.gov

One of the keys to commercialization of advanced biofuels is the development of cost-competitive ways to extract fermentable sugars from lignocellulosic biomass. The use of enzymes from thermophiles -- microbes that thrive at extremely high temperatures and alkaline conditions -- holds promise for achieving this development. Finding the most effective of these microbial enzymes, however, has been a challenge.

That challenge has now been met by a collaboration led by researchers with the U.S. Department of Energy (DOE)'s Emeryville, Calif.-based Joint BioEnergy Institute (JBEI), including Patrik D'haeseleer, a computational systems biologist who holds appointments with both JBEI and Lawrence Livermore National Laboratory (LLNL).

Working with a compost-derived consortium of thermophillic bacterium adapted to grow on switchgrass, a leading potential fuel crop, and using a combination of metagenomic and metaproteomic technologies, the collaboration has identified individual microbial species whose enzymes were the most active in deconstructing the switchgrass biomass. Major institutes in addition to JBEI participating in this collaboration included DOE's Joint Genome Institute (JGI), and EMSL, the Environmental Molecular Sciences Laboratory, a national scientific user facility at Pacific Northwest National Laboratory (PNNL).

"This marks the first time that the functional roles of individual microbial populations within a consortium have been linked with specific enzyme activities, in this case cellulase and hemicellulase," said Steven Singer, director of JBEI's microbial communities program. "Since these activities are broadly relevant to biofuel production, this is one of the first real-world applications being met by combining metagenomics and metaproteomics."

Singer, who is also a research scientist with Lawrence Berkeley National Laboratory (Berkeley Lab)'s Earth Sciences Division, is the senior author of a paper describing this research in the journal PLOS One titled "Proteogenomic Analysis of a Thermophilic Bacterial Consortium Adapted to Deconstruct Switchgrass." Co-authors are D'haeseleer, the paper's lead author; John Gladden, Martin Allgaier, Patrik Chain, Susannah Tringe, Stephanie Malfatti, Joshua Aldrich, Carrie Nicora, Errol Robinson, Ljiljana Pasa-Tolic, Philip Hugenholtz and Blake Simmons.

Advanced biofuels -- liquid transportation fuels synthesized from the sugars in cellulosic biomass -- offer a clean, green and renewable alternative to gasoline, diesel and jet fuels. However, unlike the simple sugars in corn grain, the cellulose and hemicellulose in biomass are difficult to extract in part because they are embedded in a tough woody material called lignin. Thermophilic microbes are believed to be a rich source of cellulase and hemicellulase enzymes for lignocellulosic biomass deconstruction that are active at elevated temperatures and in the presence of pretreatment chemicals such as ionic liquids.

"Natural microbial communities that deconstruct biomass, such as those found in cow rumen or compost piles, are often too complex to decipher roles for individual microbial populations," said Singer. "However, enrichment cultures established with defined substrates and at constant temperatures offer the possibility of simplifying these complex microbial communities and identifying functional roles for specific populations within the community."

As part of their efforts to develop a cost-effective way to deconstruct lignocellulosic biomass into sugars for fuel, researchers in JBEI's Deconstruction Division cultivated the switchgrass-feeding, compost-derived consortium of thermophiles.

"Using pretreated switchgrass at temperatures up to 80 degrees Celsius, we demonstrated that this consortium is an excellent source of enzymes for the development of enzymatic cocktails tailored to biorefinery processing conditions," said Blake Simmons, a chemical engineer who heads JBEI's Deconstruction Division and was a member of this research collaboration.

To identify the functional roles of community members within the switchgrass-feeding consortium, Singer, Simmons and their colleagues first used shotgun sequencing, a powerful metagenomics technique that enabled them to determine the metabolic potential of all the members of the consortium. They then used metaproteomic measurements to identify those enzymes, predicted by metagenomic analysis, that were actually produced by the microbial community.

"Doing metagenomics by shotgun sequencing is a bit like raiding a toy store and tossing hundreds of jigsaw puzzles onto a pile," D'haeseleer said.

"Each individual puzzle piece may carry some useful information, but you only start to see the bigger picture once you reassemble the pieces," D'haeseleer says. "Our collaborators at JGI used deep sequencing of millions of small pieces of DNA, and generated a partial assembly based on direct matches between the pieces. We developed a novel phylogenetic binning method to separate those partially assembled pieces into the major bacterial genomes in the consortium. This allowed us to then model the metabolic potential of all those members of the consortium."

Analysis of metagenomic sequencing data identified the most abundant microbial populations in the consortium to be closely related strains of Thermus thermophilus and Rhodothermus marinus. However, based on the assigned fractions of the switchgrass deconstruction proteome, the strains showing the most active role in switchgrass deconstruction were Gemmatimonadetes and Paenibacillus. By comparison, the more numerous Rhodothermus strain contributed fewer enzymes to biomass deconstruction.

In addition to developing a better understanding of the microbial populations that could be used for advanced biofuels, the PLOS One journal work also gleaned more information about microbial ecology.

"We live in a world that is dominated by microbial organisms all around us and we really don't understand how they work," D'haeseleer said. "We've been able to study a small fraction of these organisms in isolation, but this work helps us understand how these complex interacting communities function."

"This kind of approach can be applied to understanding how compost communities break down plant material and can give us insight into how we can design systems similar to these degradation processes to make biofuels out of plants. Our work also offers an approach to study the microbial communities involved in soil health, the health of rivers and oceans, and the functioning of the human microbiome (the bacteria in people's skin, hair and other parts of the body) inside us, D'haeseleer added.

"By leveraging the unique capabilities of the JGI and EMSL with those at JBEI, we're developing a more comprehensive functional understanding of how microbial consortia work to breakdown lignocellulose, and identifying the genes and enzymes that are responsible for this deconstruction," Simmons said. "The list of genes and enzymes generated by this study has been placed into our expression pipeline and are being used to develop optimized cocktails that are capable of generating high sugar yields from pretreated lignocellulosic biomass."

This research was supported by the DOE Office of Science.

JBEI is one of three Bioenergy Research Centers established by the DOE's Office of Science in 2007. It is a scientific partnership led by Berkeley Lab and includes the Sandia National Laboratories, the University of California campuses of Berkeley and Davis, the Carnegie Institution for Science and the Lawrence Livermore National Laboratory. DOE's Bioenergy Research Centers support multidisciplinary, multi-institutional research teams pursuing the fundamental scientific breakthroughs needed to make production of cellulosic biofuels, or biofuels from nonfood plant fiber, cost-effective on a national scale.

DOE's Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, visit the Office of Science website.


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